Instrumentation on Multi-Scaled Scattering of Bio-Macromolecular Solutions
نویسندگان
چکیده
The design, construction and initial tests on a combined laser light scattering and synchrotron X-ray scattering instrument can cover studies of length scales from atomic sizes in Angstroms to microns and dynamics from microseconds to seconds are presented. In addition to static light scattering (SLS), dynamic light scattering (DLS), small angle X-ray scattering (SAXS) and wide angle X-ray diffraction (WAXD), the light scattering instrument is being developed to carry out studies in mildly turbid solutions, in the presence of multiple scattering. Three-dimensional photon cross correlation function (3D-PCCF) measurements have been introduced to couple with synchrotron X-ray scattering to study the structure, size and dynamics of macromolecules in solution.
منابع مشابه
The Development of a Digital Multichannel Correlator System for Light Scattering Experiments
The measurement of light scattering is a well established scientific method for the analysis of particle sizes in macromolecular solutions [1]. The spectrum of applications reaches from physics, over pharmacology, molecular and cell biology, immunology right up to environmental research. The underlying principle of this approach is the extraction of spectral information from light scattering us...
متن کاملDifferently Environment Stable Bio-Silver Nanoparticles: Study on Their Optical Enhancing and Antibacterial Properties
Generally, limited research is extended in studying stability and applicational properties of silver nanoparticles (Ag NPs) synthesized by adopting 'green chemistry' protocol. In this work, we report on the synthesis of stable Ag NPs using plant-derived materials such as leaf extract of Neem (Azadirachta indica) and biopolymer pectin from apple peel. In addition, the applicational properties of...
متن کاملSmall-angle scattering studies of biological macromolecules in solution
Small-angle scattering (SAS) of x-rays and neutrons is a fundamental tool in the study of biological macromolecules. The major advantage of the method lies in its ability to provide structural information about partially or completely disordered systems. SAS allows one to study the structure of native particles in near physiological environments and to analyse structural changes in response to ...
متن کاملEffects of Polydispersity, Branching and Chain Stiffness on Quasielastic Light Scattering
Aspects of polarized coherent quasi-elastic scattering by dilute macromolecular solutions are discussed. Effects of polydispersity, branching and chain stiffness are considered, particularly in respect to the observable first cumulant of the dynamic structure factor and its dependence on the scattering angle. A combination of integrated and time-resolved scattering data yields the maximum infor...
متن کاملProbabilistic models and machine learning in structural bioinformatics.
Structural bioinformatics is concerned with the molecular structure of biomacromolecules on a genomic scale, using computational methods. Classic problems in structural bioinformatics include the prediction of protein and RNA structure from sequence, the design of artificial proteins or enzymes, and the automated analysis and comparison of biomacromolecules in atomic detail. The determination o...
متن کامل